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PCR Conventional / Qualitative PCR

- Found 11504 results

PCR Hot start PCR - Bacterial DNA Experiment

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality hot-start DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

DNA PCR Hot start PCR Bacterial DNA
PCR Multiplex PCR - Bacterial DNA Experiment

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

DNA PCR Multiplex PCR Bacterial DNA
PCR Multiplex PCR - Mammalian DNA Experiment

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

DNA PCR Multiplex PCR Mammalian DNA
PCR Quantitative real-time PCR - Experiment

DNA PCR Quantitative real-time PCR
PCR Multiplex PCR - Viral Experiment

DNA PCR Multiplex PCR Viral
PCR Quantitative real-time PCR - Viral Experiment

DNA PCR Quantitative real-time PCR Viral
PCR Multiplex PCR - Poultry DNA Experiment

DNA PCR Multiplex PCR Poultry DNA
PCR ORNi-PCR - Plasmid DNA Experiment

DNA PCR ORNi-PCR Plasmid DNA
How “strong” is the PCR product of methylation specific PCR? Discussion

Does anyone know how “strong” the PCR product of methylation specific PCR is? I kept my PCR products at 4C for about 3 weeks and then at room temperature for another week. Will I be able to use them for sequencing?

Discussions How “strong” is the PCR product of methylation specific PCR?
PCR Quantitative real-time PCR - Fish species DNA Experiment

DNA PCR Quantitative real-time PCR Fish species DNA

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